Tetraploid Induction of Hyoscyamus muticus L. using Colchicine Treatment
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چکیده
منابع مشابه
Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment
Basil (Ocimum basilicum) is one of the important medicinal plant species. In order to produce an autotetraploid population of basil (Ocimum basilicum) by colchicine, different concentrations (0.00, 0.05, 0.10, 0.20, 0.50 and 0.75%) and four treatment methods were examined (seed, the growing point of seedlings at the emergence of cotyledone leaves stage and emergence of true two type leaves stag...
متن کاملA soluble auxin-binding protein from Hyoscyamus muticus is a glutathione S-transferase.
We have used the photoaffinity label azido-[3H]IAA (5-N3-[7-3H]indole-3-acetic acid), a biologically active analog of indole-3-acetic acid, to identify auxin-binding proteins (ABPs) in the soluble fraction of Hyoscyamus muticus. A 25-kD polypeptide previously described (H. Macdonald, A. M. Jones, P. J. King [1991] J Biol Chem 266: 7393-7399) has now been purified to homogeneity by conventional ...
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This study was aimed to obtain polyploid plants of viola (Viola acuminata Ledebour) using the antimitotic agent colchicine. Experiments consisted in using colchicine on ungerminated seeds. The seeds were plunged into colchicine solutions in concentrations of 0.01, 0.05, 0.10 and 0.20% for 24 and 48 h. Polyploidy levels (diploid, tetraploid and hexaploid) were firstly detected by chromo...
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The free peritoneal fluid cells of the rat (eosinophils, mast cells, monocytic and lymphoid elements) are normally spheroidal. When allowed to settle on a solid substrate, such as a microscope slide, all but the mast cells display ameboid movements. After administration of colchicine to the animals, the morphology of the free cells collected has become altered in a characteristic manner which i...
متن کاملHeterologous expression of Vitreoscilla hemoglobin (VHb) and cultivation conditions affect the alkaloid profile of Hyoscyamus muticus hairy roots.
Fast-growing hairy root cultures of Hyoscyamus muticus induced by Agrobacterium rhizogenes offer a potential production system for tropane alkaloids. Oxygen deficiency has been shown to limit growth and biomass accumulation of hairy roots, whereas little experimental data is available on the effect of oxygen on alkaloid production. We have investigated the effect of Vitreoscilla hemoglobin (VHb...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2008
ISSN: 1028-8880
DOI: 10.3923/pjbs.2008.2653.2659